Detection of specific antinuclear antibodies (ANA) is very importance in term of diagnosis, prognosis and management of patients with systemic lupus erythematosus (SLE). To date, Line immunoassay (LIA), enzyme-linked immunosorbent assay (ELISA) and Crithidia luciliae indirect immunofluorescence assay (CLIF) are commonly used for detection of specific ANA.