Expression of a PYCARD/ASC variant lacking exon 2 in Japanese patients with palindromic rheumatism increases interleukin-1β secretion

Yumi Suganuma,¹ Hayate Tanaka,¹ Aya Kawase,¹ Aoi Kishida,¹ Moeko Yamaguchi,¹ Atsuko Yabuuchi,¹ Koji Inoue,² Shunichi Shiozawa,³ Koichiro Komai¹

¹ Division of Medical Biophysics, Department of Biophysics, Graduate School of Health Sciences, Kobe University, Kobe, Japan
² Shichikawa Arthritis Research Center, Osaka Rehabilitation Hospital, Hannan, Japan
³ Institute for Rheumatic Diseases, Nagahama, Japan

Abstract

Background: Palindromic rheumatism (PR) is a rare periodic arthritis characterized by relapsing short episodes of arthritis. Although the pathogenesis of PR is still unclear, the clinical condition is similar to that of autoinflammatory diseases caused by dysregulation of inflammasome-related genes.
Objective: We analyzed the inflammasome adapter PYD and CARD domain-containing protein/apoptosis-associated speck-like protein containing a CARD (PYCARD/ASC) in Japanese patients with PR.
Methods: Serum interleukin (IL)-1β concentrations in three Japanese patients with PR were measured. We also cloned PYCARD/ASC cDNA variants and expressed them in THP-1 cells to determine their effects on inflammasome activity following stimulation with phorbol 12-myristate 13-acetate and monosodium urate. Lysates of recombinant THP-1 cells were subjected to co-immunoprecipitation assays.
Results: Serum IL-1β concentrations were significantly elevated in patients with PR, and a splice variant of PYCARD/ASC mRNA lacking exon 2 (Δexon2) was dominantly expressed compared with that in controls. Moreover, IL-1β secretion was significantly increased in THP-1 cells expressing Δexon2 PYCARD/ASC compared with that in cells expressing the wild-type protein. The amount of NLRP3 bound to Δexon2 PYCARD/ASC was increased after stimulation, whereas that bound to the wild-type protein was decreased. There were no differences in caspase-1 binding.
Conclusion: Δexon2 PYCARD/ASC was associated with the pathogenesis of PR.
Key words: palindromic rheumatism, inflammasome, PYD and CARD domain-containing protein/apoptosis-associated speck-like protein containing a CARD, splice variant, interleukin-1β,